Hard-Level Genetic Engineering Quiz
A challenging set of multiple‑choice items covering advanced techniques and theory in genetic manipulation.
Quiz Questions
Answer all questions below and test your knowledge.
- 1
Which molecular component directly recognizes the target DNA sequence in a CRISPR‑Cas9 complex?
- 2
Base editors that convert C·G to T·A without creating double‑strand breaks belong to which class?
- 3
Which microbial host is most commonly engineered for large‑scale production of isoprenoid pharmaceuticals due to its fast growth and well‑characterized genetics?
- 4
What high‑throughput method quantifies genome‑wide off‑target cleavage of CRISPR nucleases by capturing double‑strand breaks?
- 5
A reversible gene drive designed to spread a trait temporarily relies on which genetic safeguard?
- 6
When engineering a synthetic promoter for high expression in plants, which element primarily determines transcriptional strength?
- 7
Under US law, which agency reviews investigational new drug applications for gene‑therapy clinical trials?
- 8
Prime editing combines a catalytically impaired Cas9 with which enzyme to enable precise DNA insertions?
- 9
Flux balance analysis in engineered microbes primarily predicts what?
- 10
Which principle specifically addresses the moral obligation to prevent harm when releasing genetically modified organisms into the environment?
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