Advanced Quiz on Engineered Microorganisms and Sustainable Biomanufacturing

A hard-level assessment focusing on cutting-edge applications of microbes in large-scale chemical production.

synthetic biologybiofuelsgreen chemistrymetabolic engineeringscale-upindustrial enzymesdownstream purificationupstream processingfermentationbiocatalysis
Difficulty:HARD

Quiz Details

Questions10
CategoryBiotechnology
DifficultyHARD
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Quiz Questions

Answer all questions below and test your knowledge.

  1. 1

    Which genetic modification most directly enables yeast to produce the artemisinin precursor artemisinic acid at high titers?

    Question 1
  2. 2

    In bacterial polyhydroxyalkanoate (PHA) production, how does nitrogen limitation affect carbon flux?

    Question 2
  3. 3

    For industrial secretion of cellulases to hydrolyze lignocellulose, which host organism provides the highest native secretion capacity?

    Question 3
  4. 4

    When purifying a secreted enzyme at scale using affinity chromatography, which affinity tag is most commonly employed?

    Question 4
  5. 5

    A thermotolerant lipase from Bacillus licheniformis shows optimal activity for biodiesel synthesis in which temperature range?

    Question 5
  6. 6

    During multiplex CRISPR‑Cas9 editing of Corynebacterium glutamicum to boost L‑lysine output, which element is co‑expressed to mitigate Cas9 toxicity?

    Question 6
  7. 7

    Which immobilization matrix offers high mechanical strength while allowing substrate diffusion for continuous succinic acid fermentation?

    Question 7
  8. 8

    Life‑cycle assessment of bio‑based succinic acid versus petroleum‑derived routes shows the greatest reduction in which impact category?

    Question 8
  9. 9

    In engineered Escherichia coli producing 1,3‑propanediol, which enzyme catalyzes the final reduction of 3‑hydroxypropionaldehyde?

    Question 9
  10. 10

    During scale‑up of a fed‑batch fermentation where dissolved oxygen becomes limiting, which operational change most effectively restores oxygen transfer?

    Question 10

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